Generator

Part:BBa_I712082:Design

Designed by: Marko Bitenc   Group: iGEM07_Ljubljana   (2007-10-22)


CMV promoter - CD4 (shortened)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1263
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1876
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1710
    Illegal SapI.rc site found at 798


Design Notes

Construct was amplified with CMV foward and CD4(shortened) reverse primer.


Source

Amplification from CMV-CD4-TIR.

References