Generator
Part:BBa_I712082:Design
Designed by: Marko Bitenc Group: iGEM07_Ljubljana (2007-10-22)
CMV promoter - CD4 (shortened)
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 1263
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 1876
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 1710
Illegal SapI.rc site found at 798
Design Notes
Construct was amplified with CMV foward and CD4(shortened) reverse primer.
Source
Amplification from CMV-CD4-TIR.